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KMID : 1098420210290010001
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Korean Journal of Medicinal Crop Science
2021 Volume.29 No. 1 p.1 ~ p.10
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Leaf Extract of Sedum kamtschaticum Fisch. & Mey. Ameliorates Cognitive Dysfunction in Trimethyltin-treated Rats
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Lee Seung-Eun
Ye Min-Sook
Jung Jong-Hun
Park Saet-Byul
Lee Jeong-Hoon
Kim Hyung-Don
Jang Gwi-Yeong
Seo Kyung-Hae
Kim Dong-Hwi
Shim In-Sop
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Abstract
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Background: Alzheimer’s disease (AD) is a major neurodegenerative disorder and major type of dementia with progressive cognitive deficits, including multifactorial disorders. The present study examined the therapeutic effect of Sedum kamtschaticum leaf extract (SKE) on cognitive dysfunction disorder induced in rats through trimethyltin (TMT) administration.
Methods and Results: In the preliminary screening experiment, SKE was selected as an acetylcholinesterase (AChE) inhibitor. Rats in the negative control and SKE groups were intraperitoneally injected with TMT (8 ㎎/㎏ body weight) dissolved in 0.9% saline and normal rats were injected with 0.9% saline alone. Doses of SKE (100, and 300 ㎎/㎏) were orally administered to the rats for 14 days. Normal and control rats were treated with saline for the same period. We performed a behavioral test using a Morris water maze and found that the escape and latency time periods of the SKE300 rats were shorter than those of the control rats. SKE therefore ameliorated cognitive dysfunction in this animal model. Immunohistochemical assay of biomarkers in the hippocampal cells revealed that the cell levels of phosphorylated cAMP response element binding protein (p-CREB) and AChE in the CA3 region of the control group were lower than those in the normal group. However, p-CREB and AChE reactive hippocampal cells in SKE300 group were significantly increased compared to those of the control. The hippocampal cells of brain-derived neurotrophic factor (BDNF) and protein kinase C (PKC) in control rats tended to be lower than the normal group, while the cells in the SKE groups tended to be higher than the control group.
Conclusions: These results suggest that SKE ameliorates cognitive disorders through the neuroprotective effects related to p-CREB and AChE in the hippocampal cells.
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KEYWORD
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Sedum kamtschaticum Fisch. & Mey, Acetylcholinesterase, Cognition, Phosphorylated cAMP Response Element Binding Protein, Trimethyltin
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